Biology Important Question Bank: Biotechnology Process and Applications

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1 Mark Questions

Q1. Define Biotechnology.

Q2. Who developed the first recombinant DNA?

Q3. Name any two enzymes used in r-DNA technology.



Q4. What are vectors in genetic engineering?

Q5. Give the significance of the marker gene in a vector.

Q6. What are transposons?

Q7. Define Plasmids.



Q8. Name the transposons showing copy and paste mechanism.

Q9. Name the transposons showing cut and paste mechanism.

Q10. What are sticky ends in DNA?

Q11. What are restriction fragments?

Q12. Define genomic Library.



Q13. Define c-DNA Library.

Q14. Name the three steps of PCR-technique in proper sequence.

Q15. Mention the use of Gel-Electrophoresis in biotechnology

Q16. Mention two applications of biotechnology.

Q17. Give examples of two Genetically modified plants.



Q18. Give the advantage of Golden rice.

Q19. What is biopiracy?

Q20. Name the two soil bacteria useful in biotechnology.

2 Marks Question

Q1. Define genetic engineering. Give its applications.

Q2. Considering the therapeutic products made by recombinant DNA techniques match the Column A and Column B.



Q3. Classify transposons explaining their mechanism.

Q4. Write a brief note on “transferable genetic elements”.

Q5. Write a note on DNA library prepared by “Teminism” process.

Q6. Write a note on Flavr savr tomato.



Q7. Explain how Agrobacterium tumefaciens is useful in biotechnology.

Q8. Mention the basic requirements needed for PCR technique.

3 Marks Questions

Q1. Recombinant DNA technology deals with manipulating the genome of a cell or organism to obtain a desirable phenotype. Enlist the basic steps involved in this process. (Steps used in r-DNA technology)

Q2. What are restriction fragments? How are these analyzed, mention their uses?

Q3. Restriction endonucleases make cut on some specific sites of DNA molecule. Give an account
of such sites using a suitable example?

Q4. A soil bacterium that was traditionally used as an insecticidal spray is now being used in the production of insect-resistant GM crops. Give a brief account of this bacteria and its application in biotechnology.



Q5. Explain the terms bio-piracy and bio-patent giving examples.

Q6. What is DNA Library? Describe how a c-DNA Library is constructed.

Q7. Explain nomenclature procedure of restriction endonucleases giving a suitable example.

Q8. There are some sequences of DNA which can change their position within the genome of a single cell. Give an account of such DNA sequences.



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One Comment

  1. 1mark question
    Q.1) Biotechnology is a branch of biology which deals with the techniques of using live organisms, enzymes or biological processes to develop products and provide services for human welfare

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